Journal: Scientific Reports

Article Title: Revisiting single cell analysis in forensic science

doi: 10.1038/s41598-021-86271-6

Figure Lengend Snippet: Locus-specific peak heights (RFU) vs. cycle number for the CSF1PO, Penta D, and SE33 loci amplified using the Promega PowerPlex Fusion 6c kit.

Article Snippet: All samples were amplified on the Life Technologies Veriti thermal cycler using the Promega PowerPlex Fusion 6c human DNA amplification kit (DC2705).

Techniques: Amplification

Journal: Scientific Reports

Article Title: Revisiting single cell analysis in forensic science

doi: 10.1038/s41598-021-86271-6

Figure Lengend Snippet: Average proportions of allele dropout by locus for single cells at 29, 30 and 31 cycles using the PowerPlex Fusion 6c kit analyzed at ( a ) 10 RFU, ( b ) 100RFU and ( c ) 150RFU. Cycle numbers within each locus from left to right are 29, 30, and 31, respectively and the colors (blue, green, yellow, red and purple) represent the dye channel.

Article Snippet: All samples were amplified on the Life Technologies Veriti thermal cycler using the Promega PowerPlex Fusion 6c human DNA amplification kit (DC2705).

Techniques:

Journal: Scientific Reports

Article Title: Revisiting single cell analysis in forensic science

doi: 10.1038/s41598-021-86271-6

Figure Lengend Snippet: Average proportions of locus dropout by locus for single cells at 29, 30 and 31 cycles using the PowerPlex Fusion 6c kit analyzed at ( a ) 10 RFU, ( b ) 100RFU and ( c ) 150RFU. Cycle numbers within each locus from left to right are 29, 30, and 31, respectively and the colors (blue, green, yellow, red and purple) represent the dye channel.

Article Snippet: All samples were amplified on the Life Technologies Veriti thermal cycler using the Promega PowerPlex Fusion 6c human DNA amplification kit (DC2705).

Techniques:

Journal: Scientific Reports

Article Title: Revisiting single cell analysis in forensic science

doi: 10.1038/s41598-021-86271-6

Figure Lengend Snippet: Random match probabilities (calculated without theta corrections and the use of the “2p” rule) for profiles generated from single cells amplified with the PowerPlex Fusion 6c amplification kit using 29, 30, and 31 cycles and analyzed using analytical thresholds of 100 and 150RFU.

Article Snippet: All samples were amplified on the Life Technologies Veriti thermal cycler using the Promega PowerPlex Fusion 6c human DNA amplification kit (DC2705).

Techniques: Generated, Amplification

Journal: Scientific Reports

Article Title: Revisiting single cell analysis in forensic science

doi: 10.1038/s41598-021-86271-6

Figure Lengend Snippet: Locus-specific mean peak heights for single cells amplified using 29, 30 and 31 cycles and the PowerPlex Fusion 6c human DNA amplification kit.

Article Snippet: All samples were amplified on the Life Technologies Veriti thermal cycler using the Promega PowerPlex Fusion 6c human DNA amplification kit (DC2705).

Techniques: Amplification, DNA Amplification

Journal: Forensic Science International: Synergy

Article Title: Interpol review of forensic biology and forensic DNA typing 2016-2019

doi: 10.1016/j.fsisyn.2019.12.002

Figure Lengend Snippet: Summary of rapid DNA instrument validation and evaluation studies published from 2016 to 2019.

Article Snippet: Commercially available rapid DNA instruments include (1) the DNAscan/ANDE 4C (4-color) now called ANDE 6C (Accelerated Nuclear DNA Equipment 6-color) Rapid DNA System, originally developed by NetBio (Waltham, MA), which has become ANDE (Longmont, CO) ( https://www.ande.com/ ), and (2) the RapidHIT 200 and (3) RapidHIT ID instruments, originally developed by IntegenX (Pleasanton, CA) and now sold by Thermo Fisher Scientific (South San Francisco, CA) (see https://thermofisher.com/rapidDNA ).

Techniques: Inhibition, Electrophoresis, Software